Conditioned enhancement of antibody response against influenza virus hemagglutinin using camphor odor as conditioned stimulus in mice

VernacularTitle:樟脑气味嗅觉条件刺激诱发小鼠条件反射性抗体增强的实验研究
Author: Meng SUN ; Xiaoli QI ; Wenjuan LIN
Publication Type:Journal Article
Keywords: Conditioned enhancement of antibody; Influenza virus hemagglutinin; Camphor odor
From: Chinese Journal of Behavioral Medicine and Brain Science 2009;18(4):295-296
CountryChina
Language:Chinese
Abstract: Objective To observe conditioned enhancement of antibody production against influenza vaccine. Methods 36 female BALB/c mice were injected with 3 μg/mouse influenza virus hemagglutinin (HA) as the unconditioned stimulus (UCS),and camphor odor was served as the conditioned stimulus (CS). After a CS/UCS pairing was made,animals were re-exposed to the CS at Weeks 6. Results Through one conditioned stimulus,the optical density of anti-HA antibody of the conditioned group (Weeks 9:0.68±0.06; Weeks 10:0.60±0.06)was significantly increased compared with the unconditioned group (Weeks 9:0.53±0.06; Weeks 10:0.48±0.04) ( P <0.01). The level of anti-HA antibody of the conditioned group was also significantly greater than other controlled groups( P <0.05). Conclusion Through a single exposure to camphor odor which was paired with immunization of influenza virus HA in a single trial learning protocol,a significant conditioned anti-HA IgG production occurred.