Molecular mechanism of different sensitivities of PML-RARα to apoptosis induced by apoptosis inducing agents
10.3760/cma.j.issn.1673-422X.2009.05.023
- VernacularTitle:PML-RARα表达对细胞凋亡诱导剂敏感性差异的分子机制研究
- Author:
Haitao ZHAO
;
Peie WEN
;
Xia REN
;
Weihua YANG
;
Hua FAN
;
Gaojuan QIAO
;
Tianhua TANG
;
Haiquan REN
;
Kehong BI
;
Guosheng JIANG
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Arsenicals
- From:
Journal of International Oncology
2009;36(5):394-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular mechanism of different sensitivities to apoptosis induced by low concentration of As2O3 in PML-RARα negative HL-60 cells and PML-RARα positive NB4 cells. Meth-ods NB4 and HL-60 cells were cultured with As2O3 for 1 to 4 days; cell proliferation were detected by MTT method; the apoptosis was detected by flow cytometry,Bcl-2,Bax and Fas mRNA were determined by RT-PCR. Results The proliferation of NB4 cells was inhibited obviously by As2O3(1.0 μmol/L)with the induction of apoptosis( P <0.05) ,which was accompanied by the down-regulation of Bcl-2 mRNA expression( P <0.05)and the ratio of Bcl-2/Bax(P <0.05), but there was no obvious variation of Bax and Fas expression( P >0.05). Inhibition of proliferation and apoptosis were not obvious in PML-RARα negative HL-60 cells induced by low concentration As2O3 ( P >0.05), and there was no obvious variation of Bcl-2, Bax, Fas mRNA expres-sion or Bcl/Bax ratio( P >0.05). Conclusion The ratio of Bcl-2/Bax is contributed to the different sensitiv-ities of PML-RARα negative HL-60 cells and positive NB4 cells induced by low concentration of As2O3.
