Effect of rAAV2-TGFβ3 on collagen degradation of liver fibrosis in rats
10.3760/cma.j.issn.1674-2397.2009.02.008
- VernacularTitle:rAAV2-TGFβ3对肝纤维化大鼠肝脏组织胶原降解的影响
- Author:
Ping LIU
;
Xiaoliang GAO
;
Wei QIAN
;
Keshu XU
- Publication Type:Journal Article
- Keywords:
Liver cirrhosis;
Transforming growth factor β3;
Matrix metallopmteinase;
Recombinate adeno-associated virus
- From:
Chinese Journal of Clinical Infectious Diseases
2009;2(2):89-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effect of transforming growth factor β3(TGFβ3)on expression of MMP-9,MMP-2,TIMP-1 and collagen I in rats with liver fibrosis through transduction by recombinant adeno-associated virus 2(rAAV2).Methods Rats were randomly divided into 4 groups:normal control group,model group,negative control group and TGFβ3 group.Liver fibrosis model was induced by hypodermic injection of 40% CCl4.rAAV2-TGFβ3,was injected via vena caudalis of the rats one week before CCl4 Was given.All rats were sacrificed and the liver tissues were taken 8 weeks afler injection of CCl4.The histopathological changes were observed on HE sections;the expressions of MMP-9,MMP-2,TIMP-1 and collagen I were examined by histochemistry and the positive area rates were semi-quantitatively analyzed.Results Compared with the model group and negative control group,the decreases of inflammatory infiltration and collagen fibers hyperplasia were observed in the TGFβ3 group,and the expression of MMP-9 increased(q=23.664,27.746,P<0.01),the expression of collagen I(q=5.503,5.251,P<0.01)and TIMP-1(q=5.800,8.608,P<0.01)decreased,but that of MMP-2 was not changed(q=2.1 08,0.996,P>0.05).Conclusion rAAV2-TGFβ3 can reduce the histological damage and degree of liver fibrosis in rats by inhibiting expression of TIMP-1 and collagen I,and upregulating expression of MMP-9.
