The distribution of 16S rRNA methylase genes in KPC-producing Klebsiella pneumoniae strains
10.3760/cma.j.issn.1674-2397.2009.02.003
- VernacularTitle:16S rRNA甲基化酶在产KPC酶肺炎克雷伯菌中的分布
- Author:
Liying LU
;
Weili ZHANG
;
Qing YANG
;
Hua ZHOU
;
Yunsong YU
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
RNA methylase;
Carbapenenase
- From:
Chinese Journal of Clinical Infectious Diseases
2009;2(2):71-73
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of 16S rRNA methylase genes in Klebsiella pneumoniae strains producing Klebsiella pneumoniae ealbapenenase type 2(KPC-2).Methods A total of 37 Klebsiella pneumoniae isolates producing KPC-2 were collected.The minimal inhibitory concentrations (MICs)of these strains to amikacin,gentamyein and netilmicin were determinated by agal dilution method.Six 16S rRNA methylase genes(armA,rmtA,rmtB,rmtC,rmtD and npmA)were detected by PCR.Results The resistant rates to amikacin,gentamycin and netilmicin were 97.3%(MIC50≥1024μg/mL).Among those resistant strains,8 were armr/A positive,25 were rmtB positive,4 were both armA and rmtB positive.and no other 16S rRNA methylase genes were found.The total positive rate of 16S rRNA methylase genes was 78.4%(29/37).Conclusion 16S rRNA methylase genes armA and rmtB ale prevalent in Klebsiella pneumoniae strains producing KPC-2.
