Effect of ultrasound irradiation combined with liposome membrane microbubbles on the reorgnization of cytoskeleton in vascular smooth muscle cells
10.3760/cma.j.issn.1004-4477.2009.05.027
- VernacularTitle:超声辐照脂质膜微泡对血管平滑肌细胞骨架组装的影响
- Author:
Ping ZHANG
;
Yunhua GAO
;
Zheng LIU
;
Ping LIU
;
Kaibin TAN
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Aorta,thoracic;
Myocytes,smooth muscle;
Cytoskeleton
- From:Chinese Journal of Ultrasonography
2009;18(5):444-447
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ultrasound irradiation combined with liposome membrane microbubbles on the reorgnization of cytoskeleton in vascular smooth muscle cells (VSMCs). Methods Rat thoracic aortic VSMCs were cultured in vitro. VSMCs were exposed to 1 MHz continuous waves ultrasound radiation for 120 s at intensity 0.3 W/cm2in the presence of liposome membrane microbubbles (1 μl/ml) after treated with platelet derived growth factor-BB (PDGF-BB). The reorganizations of microfilaments, microtubules and intermediate filaments were examined by using immunofluorescence and fluorocytochemistry techniques. Results There was a substantial increase in the expression of F-actin and assembly of long bundles of stress fibers in the transversed cell body when treated with PDGF-BB. Neither alterations of β-tubulin nor of vimentin cytoskeletal protein organization were observed in PDGF-BB treated cells as compared to those of the contol group. After ultrasound irradiation combined with liposome membrane microbubbles, the expression of F-actin, β-tubulin and vimentin were reduced along with the simultaneous changes in microfilaments, microtubles and intermediate filaments array. Conclusions Ultrasound irradiation combined with liposome membrane microbubbles can induce significant changes in cytoskeleton structure of VSMCs cultured in vitro.
