Expression profile in the liver of rats with idiopathic hyperoxaluria

VernacularTitle:特发性高草酸尿症大鼠肝脏差异表达基因的筛选研究
Author: Ding XIA ; Zhiqiang CHEN ; Xuan ZHU ; Zhangqun YE
Publication Type:Journal Article
Keywords: Idiopathic hyperoxaluria; Gene expression chips
From: Chinese Journal of Urology 2009;30(6):415-418
CountryChina
Language:Chinese
Abstract: Objective To investigate differentially expressed genes in the liver of rats with idio-pathic hyperoxaluria by cDNA microarray. Methods Three male rats with idiopathic hyperoxaluria were set as study group, 3 normal ones as control. Total RNA was isolated by one-step protocol from the liver of rats in the 2 groups and mRNA was purified, reversely transcribed, labeled with Cy3 or Cy5 and hybridized to gene chip containing 26 962 target genes of rat. The differences in gene expres-sion profile between the 2 groups were analyzed by bioinformatics methods. Results Comparative a-nalysis revealed that the expression of 123 genes was up-regulated and 24 genes was down-regulated by two-fold in the liver. These genes were associated with cell receptor, immune, cell signal transduc-tion, amino acid metabolism, protein translation, cell proliferation and others. Conclusion cDNA microarray could be used effectively to screen differentially expressed genes in the liver of rats with idi-opathic hyperoxaluria in which many different kinds of genes were involved.