Application of two-round PCR in rapid molecular diagnosis of fungus-infected clinical specimens
10.3760/cma.j.issn.0412-4030.2009.06.008
- VernacularTitle:二次PCR用于临床标本真菌感染快速分子诊断的研究
- Author:
Xuelian Lü
;
Zehu LIU
;
Yaning MEI
;
Xiaoli ZHANG
;
Weida LIU
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction;
Mycoses;
Molecular diagnostic techniques
- From:
Chinese Journal of Dermatology
2009;42(6):390-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a diagnosis method for fungal infection using two-round PCR,and to evaluate its sensitivity in the detection of clinical specimens suspected to be infected with fungi.Methods A total of 29 specimens of clinical sputum and alveolar wash solution were collected from patients with suspicious fungal infection.All specimens uaderwent direct microscopy with 10%KOH,fungal culture,one-round PCR and two-round PCR.The fungal universal primer targeting ITS regions of rDNA was used in PCR.The detection rate for fungi was compared between these methods.Results The detection rate for fungi was 20.69%by direct microscopy,37.9%by fungal culture,17.2%by one-round PCR,and 48.3%by two-round PCR.More than one species of fungus were detected in 6.9%(2/29),3.4%(1/29)and 24.1%(7/29)of these specimens by fungal culture.one-round PCR and two-round PCR, respectively.There was a significant difference in the detection rate between two-and one-round PCR(x2=6.34,P＜0.05).With regard to the detection rate for more than one species of fungus,two-round PCR was significantly higher than one-round PCR and fungal culture(x2=4.09,6.30.bom P＜0.05).Conclusion Two-round PCR may help to improve the sensitivity of molecular diagnosis of fungus-infected specimens.
