Expression of inducible nitric oxide synthase and level of nitric oxide in HaCaT cells after UVA irradiation
10.3760/cma.j.issn.0412-4030.2009.06.010
- VernacularTitle:长波紫外线照射对HaCaT细胞iNOS/NO表达的影响
- Author:
Ji LI
;
Wei LI
;
Hongfu XIE
;
Xiao CHEN
;
Xiang CHEN
;
Mingliang CHEN
- Publication Type:Journal Article
- Keywords:
Ultraviolet rays;
HaCaT cells;
Nitric oxide synthase;
Nitric oxide
- From:
Chinese Journal of Dermatology
2009;42(6):396-398
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of UVA irradiation on the expression of inducible nitric oxide sgnthase(iNOS)and level of nitric oxide(NO)in HaCaT cells.Methods HaCaT cells were cultured,irradiated with difrerent doses(1,5,1 0 J/cm2)of UVA.After another 24-,48-,72-hour culture,the mRNA and protein expressions of iNOS and level of NO wen measured bv RT-PCR,Westem blot and Griess reagent kit,respectively.Results Atier the irradiation with 1.5 or 10 J/cm2 of UVA,the mRNA expression of iNOS was observed in HaCaT cells at 24 h,reached a peak at 48 h,then decreased at 72 h;significant differences were fcIund between the three time points (all P＜0.05).No expression of iNOS protein was detected in HaCaT cells at any time point after irradiation witll UVA Of 1 J/cm2;meanwhile,with UVA irradiation of 5 and 10 J/cm2,the protein expression of iNOS increased at 24 h,peaked at 48 h,but was undetected at 72 h.In HaCaT cells irradiated with UVA of 1.5 or 10 J/cm2.the level Of NO showed an increase at 24 h.a marked increase at 48 h,and a stable increase at 72 h,and significant differences were noticed between irradiated cells and control cells at three time points(all P＜0.05).In unirradiated HaCaT cells,no expression of iNOS mRNA or protein was observed with a low level of NO.Conclusions The changes in iNOS expression and NO level in HaCaT cells are related to the duration and dose of UVA irradiation.
