Tnhibitory effect of Listeria monocytogenes expressing melanoma-inhibiting activity gene on malignant melanoma
10.3760/cma.j.issn.0412-4030.2009.06.011
- VernacularTitle:携带MIA基因单核细胞增生性李斯特菌抑制恶性黑素瘤的研究
- Author:
Yue QIAN
;
Ping JIANG
;
Shujuan CHU
;
Siyuan CHEN
;
Aiping FENG
;
Qin LUO
- Publication Type:Journal Article
- Keywords:
Melanoma;
Listeria monocytogenes;
Arrestin;
Models,animal
- From:
Chinese Journal of Dermatology
2009;42(6):399-401
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effects of L. monocytogenes expressing melanomainhibiting activity(MIA)gene on malignant melanoma.Methods The plasmid pERL3-hly-MIA was constructed and used to transform live L.monocytogenes by electroporation.Atier culture.the transformed L.monocytogenes,namely LM-hly-MIA,was harvested for the detection of MIA protein by Westem Blot.A total of 24 mice were divided into three groups,namely control group,LM group and LM-hly-MIA group,to be immunized with physiological saline,L.monocytogenes and LM-hly-MIA,respectively.One day after the immunization.mice were subcutaneously inoculated with 0.1 mL of B16 cells at a concentration of 1×107/mL.One week later.reimmunization was performed.The size and weight of tumors were observed and measured in these mice.Results Enzyme digestion and Western blot confirmed the Successful construction of plasmid pERL3-hly-MIA.The average weight of tumors in mice was 4.33±0.91 gram.3.36 ±0.41 gram and 1.89±0.52 gram,respectively in the control group,LM group and LM-hly-MIA group,respectively(P＜0.05).with the inhibition rates of tumor growth being 22.4%and 61.5%in LM group and LM-hly-MIA group.respectively.Conclusion The attenuated strain of L.monocytogenes expressing MIA gene could evidently inhibit melanoma growth.
