Study on the application of solid phase extraction for quantitation vitamin D in Patarvit syrup by HCLC
- Author:
Thai Duy Thin
- Publication Type:Journal Article
- Keywords:
Vitamin D, patarvit
- MeSH:
Vitamin D;
Pharmaceutical Preparations
- From:Pharmaceutical Journal
2005;354(10):17-19
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
The study built process of extraction vitamin D3 in Patarvit syrup by SPE technique with mean extract performance as 98.2%. Chromatography was built to quantity vitamin D3 as following: Lichrosorb RP 18 column (250 X 4 mm, 5 and 10µm ); Detector UV: 265 nm; movement phase: Methanol-ethyl acetate - water (90:7:3); stream speed: 1.5ml/minute. Injection volume: 20µl. The method had a high accuracy (E% = 1.18%). Percentage of withdrawal was 96.8% with E% = 0.71%; the limit of detection (LOD) was 5.79IU/ml, the limit of quantitative (LOQ) was 19.3IU/ml
