Clinical application of detection for total core antigen of hepatitis C virus
- VernacularTitle:丙型肝炎病毒总核心抗原测定方法及临床应用
- Author:
Deming TAN
;
Dongsong NIE
;
Xiaohu PENG
;
Xiqin YANG
;
Kai LI
;
Xiuyin ZHAO
;
Yangyi OU
;
Limin ZENG
;
Songhui ZHOU
;
Heqiu ZHANG
;
Jianyuan ZHOU
- Publication Type:Journal Article
- Keywords:
Hepacivirus/AN;
Hepatitis c antigens/AN
- From:
Journal of Chinese Physician
2009;11(5):591-593
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop the technique to detect total core antigen of HCV(Total HCV-cAg) by Enzyme-Linked Immu-nosorbent Assay (ELISA) and apply it for clinical diagnosis. Methods 201 serum samples with anti-HCV antibody were detected total HCV-cAg after pre - treating the samples, then the sensitivity of results were compared with HCV RNA tests. Among them, 176 cases was determined by FQ-PCR, and 25 cases by RT-PCR for HCV-RNA. Results HCV RNA was found in sera from 88 of 201 samples (43.8%). Total HCV-cAg was positive in 71 (35.3%) of 201 samples . There was no significant difference between the detection rate of HCV RNA by PCR and total HCV-cAg by ELISA. Conclusion Detection of total core antigen of HCV is suitable to be used as to diagnose HCV in clinic.
