Expression and function of cysteinyl leukotriene receptors on human keratinocytes
10.3760/cma.j.issn.0412-4030.2009.06.012
- VernacularTitle:人角质形成细胞半胱氨酰白三烯受体的表达和功能研究
- Author:
Hua ZHONG
;
Zhifang ZHAI
;
Rushan XIA
;
Ying ZHANG
;
Baiyu ZHONG
;
Shuqian TANG
;
Fei HAO
- Publication Type:Journal Article
- Keywords:
Keratinocytes;
Leukotriene D4;
Receptors,leukotriene;
Leukotriene antagonists
- From:
Chinese Journal of Dermatology
2009;42(6):402-405
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression and function of cysteinyl leukotriene receptors (CysLTRs)in keratinocytes.Methods Human keratinocytes were isolated from the tissue of foreskin by digestion with dispase Ⅱ and trypsin,and subjected to primary culture.By using confocal laser scanning microscopy and reverse transcriptase PCR,the localization and expression of CysLTRs were studied in kemtinocytes.respectively.Some primarily cultured keratinocytes were pretreated with leukotriene D4 (30 nmoi/L),MK571(300 nmol/L),and BAYu9773 for 5 minutes followed by the detection of intmcellular calcium level using the Ca2+ indicator dye Fura-2/AM as well as cell proliferation bv MTT assay.Results The expressions of CysLTR1 and CysLTR2 were observed in cultured keratinocytes,and they were mainly located on cell membrane,partly in cytoplasm and nuclei.Compared with non.stimulated cells,a significant increase Was noted in the expression of CysLTRs,especially in the nuclei of keratinocytes stimulated by LTD4(P＜0.05),together with an elevation in intracellular calcium level(42.27±3.00 mmol/L,P＜0.01)and acceleration in cell proliferation (P＜0.01).However,both MK571 and BAYu9773 could completely block the effect of LTD4 on intmcellular calcium level and cell proliferation.and there was no significant difference in the blocking effect between MK571 and BAYu9773.Conclusions Functional CysLTRs are expressed in human keratinocytes.and they carl increase the intracellular calcium level in,and cell proliferation of,keratinocytes.
