The association between antigen-specific cytotoxic lymphocytes response and different clinical status in patients with hepatitis B
10.3760/cma.j.issn.1000-6680.2009.05.009
- VernacularTitle:乙型肝炎病毒特异性细胞毒性T淋巴细胞应答与不同临床感染状态的关系
- Author:
Yufeng WAN
;
Yaping HAN
;
Jun LI
;
Lianhua KONG
;
Shuang LI
;
Li DONG
;
Nian CHEN
;
Yuan LIU
;
Zuhu HUANG
- Publication Type:Journal Article
- Keywords:
T-lymphocytes,cytotoxic;
HLA antigens;
Hepatitis B;
Enzyme-linked immunosorbent spot;
Hepatitis B virus
- From:
Chinese Journal of Infectious Diseases
2009;27(5):287-291
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze human leucocyte antigen (HLA)-A0201 restricted antigen-specific cytotoxic lymphocytes (CTL), and to investigate the difference of T cell response to specific antigen epitopes between patients with acute phase of acute hepatitis B and active phase of chronic hepatitis B. Methods Peripheral blood mononuclear cells (PBMC) from 5 patients with acute phase of acute hepatitis B and 6 patients with active phase of chronic hepatitis B were isolated. The numbers and functions of CD8+ T-lymphocyte epitope peptide specific CTL were detected using enzyme-linked immunosorbent spot (ELISPOT) assay, and the 3 peptides were from HBV polymerase region (Pol575-583), envelope region (Env348-357) and core region (Core18-27), respectively. The data were analyzed using t test. Results The spot formation cell counts (SFC) of Pol575-583, Env348-357 and Core18-27 stimulations in patients with acute phase of acute hepatitis B were 110±13, 165±17 and 185±20, respectively; and those in patients with active phase of chronic hepatitis B were 22±4, 23±5 and 30±5, respectively; the differences were all significant (t=10.9, 15.2 and 8.0, respectively, all P<0.05). The CTL responses to the three peptides in patients with acute phase of acute hepatitis B were Pol575-5830.05). The SFC were increased upon non-antigen specific HLA-A2404 restricted epitope (Core117-125), but the difference was not significant compared with negative control group (P>0.05). Conclusions Hepatitis B virus-specific CTL responses in patients with acute hepatitis B are significantly higher than those in patients with chronic hepatitis B. The number and function of polyclonal CTL are both impaired in patients with chronic hepatitis B.
