Preliminary research on 16 peripheral blood monocyte immune rejection relative genes expression after heart transplantation with fluorescent quantitation RT-PCR techniques
10.3760/cma.j.issn.1008-6315.2009.06.003
- VernacularTitle:荧光定量RT-PCR实时检测心脏移植患者外周血单核细胞16种免疫排斥基因整体表达变化的初步研究
- Author:
Haibo ZHANG
;
Xu MENG
;
Jie HAN
;
Yixin JIA
;
Yan LI
;
Yan CHEN
;
Weijun CHEN
- Publication Type:Journal Article
- Keywords:
Peripheral blood mononuclear cell;
Heart transplantation;
Immune rejection;
Gene ex-pression
- From:Clinical Medicine of China
2009;25(6):568-572
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the micro-invasive immune rejection monitoring methods with peripher-al blood mononuclear cell gene expression detection and evaluate the clinic rejection estimation value. Methods The SYBR Green Ⅰ was used as fluorescent dye and the GAPDH as house keeping gene control in the quantitatiun RT-PCR technique to observe the 16 immune rejection relative genes expression features after heart transplantation. results were also compared with that of the normal people. Results The 16 immune rejection relative genes expres-sion were no different between normal people and the transplantation recipients before surgery (P>0.05). After heart transplantation the expression of ITGA4, FKB, ILI R-2 up regulated and the level of PF4、ITGAM、TGFβ1、 RHOU down regulated. The results were similar with the clinic observation that the immune rejection often occurs in the first 3 months after heart transplantation. It implied that these 7 genes may play an important role in the acute im-mune rejection after transplantation. Conclusion The real time quantitation RT-PCR methods were constructed suc-cessfully to detect the multiple immune relative genes expression and is of chnic applicable.
