Study on the orient differentiation from embryo hepatic Sca-1+ cell to hepatic cell under the induction of HGF and aFGF in vitro
10.3760/cma.j.issn.1008-6706.2009.05.001
- VernacularTitle:肝细胞生长因子与酸性成纤维细胞生长因子体外诱导胚胎肝干细胞抗原阳性细胞向肝细胞定向分化的研究
- Author:
Zhidong QIU
;
Huilai MIAO
;
Shenghuang ZHANG
;
Nianping CHEN
;
Ming CHEN
;
Shuhua ZHENG
- Publication Type:Journal Article
- Keywords:
Stem;
Hepatocyte growth factor;
Fibroblast Grouth Facfors
- From:
Chinese Journal of Primary Medicine and Pharmacy
2009;16(5):769-771
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasbility of the orient differentiation from embryo hepatic Sca-1+ cell to hepatic cell or hepatic cell precursor under the induction of hepatocyte growth factor(HGF) and acor fibroblast growth factor(aFGF) in vitro. Methods Sea-1+ cells were isolated by immunomagnetic beads and their morphous were observed by contrast phase microscope. The expression of mRNA of albumin(ALB) and meta-thyroprotein were detected by RT-PCR; ALB, AFP, CK8/18 protein were detected by immunohistochemical method, and cell staining for glycogen and urea synthesis function were tested. Results The activity, purity, recovery rate of Sca-1+ cells were (94. 24±1.04) %, (85.57±1.66) %, (62. 31±1.85 ) % respectively. After the induction of HGF and aFGF, Sca1+ cells became anomalism and transformed to heptic cell in morphous,disappeared in expression of AFP protein and upregulated in expression of ALB and CK8/18 protein,upregulated in expression of ALB and transthyroprotein mRNA,cell staining of glycogen and urea synthesis function were strengthened,the cell differentiated to mature hepatic cell. Conclusion Embryo hepatic Sca-1+ cell could differentiate to hepatic cell or hepatic cell precursor under the induction of HGF and aFGF in vitro.
