The effect of fibronectin and arginine-glycine-aspartic-serine tetrepeptide on chemotherapy sensitivity of liver carcinoma cell lines
- VernacularTitle:纤维连接蛋白及精-甘-天冬-丝氨酸多肽对肝癌细胞化疗敏感性的影响
- Author:
Zhongming WU
;
Weilin WANG
;
Jing JIN
;
Haiyang XIE
;
Lin ZHOU
;
Shusen ZHENG
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular;
Fibrin;
Arginine-Glycine-Aspartic-Sefine tetrepeptide
- From:
Chinese Journal of General Surgery
2008;23(7):537-539
- CountryChina
- Language:Chinese
-
Abstract:
Objecfive To study the effect of fibronectin(FN)and arginine-glycine-aspartateserine tetrapeptide (RGDS)on chemotherapy sensitivity of hepatocellular carcinoma(HCC)cell lines.Methods A HCC cell lines 7721 was divided into BSA group(BO),FN group(FO),RGDS group(RO)and FN+RGDS group(FR).Mitomycin-C(MMC)was added into each group with different concentrations for 2 h,12 h and 24 h.MTT assay was used to detect the survival rate of the tumor cell at different time,and fluorescent staining method and flow cytometry was used to detect the tumor cell's apoptosis.Results The survival rate and apoptosis rate was significantly different between each group with the treatment of MMC for 12 h or 24 h,and no statistical difference was found after incubation for 2 h.Conclusions With time FN down-regulates MMC induced apoptosis of the HCC cells.making HCC cells 1ess sensitive to chemotherapy,while RGDS enhances the sensitivity of HCC cells to chemotherapy.
