Liver graft regeneration after liver transplantation in IL-6 knockout mice
- VernacularTitle:白介素6基因敲除小鼠肝脏移植后的肝脏再生研究
- Author:
Guodong WANG
;
Yi MA
;
Guihua CHEN
;
Xiaoshun HE
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Mice;
Interleukin-6;
Gene knockout;
Regeneration
- From:
Chinese Journal of General Surgery
2008;23(7):507-509
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe recipient survival time and liver graft regeneration in an orthotopic liver transplantation model using IL-6 knockout mice as a donor.Methods A model of liver transplantation in C57BL/6 WT and IL-6 KO mice with C57BL/6 background was established.Thirty eight mice were divided into three groups:C57BL/6 wild type→C57BL/6 wild type control group(n=10),IL-6 KO→IL-6 KO group(n=14)and IL-6 KO→C57BL/6 WT group(n=14).Hepatocyte replication with BrdU uptake after liver transplantation was examined by immunohistochemistry.Resuits The survival time in the control group was＞16 days.and that was 2 days and 1.6 days in the IL-6 KO→IL-6 KO and IL-6KO→C57BL/6 WT,respectively.The difierence in survival time in all three groups was statistically significant(F=190.09,P＜0.01).The liver grafts in control group showed minimal injury and no necrosis and mild increase of BrdU uptake at 48 hours.Patchy areas of necrosis and hepatocyte ballooning were observed in the two groups using IL-6 KO mice as donors,there was minimum increase in BrdU uptake at 48 hours post transplantation.Conclusion IL-6 KO liver grafts fail to regenerate after liver transplantation.IL-6 is an important factor in liver regerative response.
