Effect of microbubbles mediated ultrasound insonation on proliferation and apoptosis of vascular smooth muscle cells in different phases of ceil cycle
10.3760/cma.j.issn.1004-4477.2009.06.025
- VernacularTitle:超声联合微泡对不同细胞周期血管平滑肌细胞增殖和凋亡的影响
- Author:
Ping ZHANG
;
Yunhua GAO
;
Ping LIU
;
Zheng LIU
;
Kaibin TAN
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Myocytes,smooth muscle;
Cell proliferation;
Apoptosis
- From:
Chinese Journal of Ultrasonography
2009;18(6):533-536
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of microbubbles mediated ultrasound insonation on proliferation and apoptosis of vascular smooth muscle cells (VSMCs) in different phase of cell cycle. Methods Rat thoracic aortic VSMCs were cultured in vitro by the method of tissue adherence. The cells were synchronized by the methods of serum starvation and double thymidine block. The synchronization results were detected by flow eytometer. VSMCs in different phases of cell cycle were exposed to 1 MHz continuous waves ultrasound for 120 s at intensity 0.3 W/cm2 in the presence of lipid-coated microbubbles (1 ml/L). Apoptosis of VSMCs was analyzed by AnnexinV/PI staining using flow eytometry. The proliferation and the proliferating cell nuclear antigen(PCNA) protein expression of VSMCs were detected by MTT assay and immunoeytochemistry, respectively. Results The synchronized G0/G1 and S phase VSMCs were achieved, with synchronized rates to 89.53 % and 66.87 %, respectively. Ultrasound sonication for 120 s with microbubbles could significantly inhibit the proliferation and downregulate the PCNA expression of S phase VSMCs,but the proliferation and PCNA expression of G0/G1 phase VSMCs were not affected. After treatment of ultrasound with microbubbles, the apoptotic ratio were found to reach (7.05 ± 2.04)% in G0/G1 phase VSMCs and (27.01 ±3.87)% in S phase VSMCs. Conclusions Microbubbles mediated ultrasound insonation can significantly inhabit the proliferation and induce apoptosis in VSMCs at proliferation stage.
