TNF-α up-regulates protein tyrosine phosphatase 1B via NF-kB in HepG2 cells

VernacularTitle:肿瘤坏死因子α通过NF-kB上调HepG2细胞中蛋白酪氨酸磷酸酶1B的表达
Author: Jie WU ; Dajin ZOU ; Miao WANG ; Xianling GUO ; Lijuan ZHANG ; Hong WU ; Bin LU ; Yue CHEN
Publication Type:Journal Article
Keywords: Protein tyrosine phosphatase 1B; Tumor necrosis factor-α; HepG2 cells; NF-kB
From: Chinese Journal of Endocrinology and Metabolism 2009;25(3):326-328
CountryChina
Language:Chinese
Abstract: HepG2 ceils were treated with various concentrations of tumor necrosis factor-or (TNF-α) for 24 hours. RT-PCR and Western blot were used to measure protein tyrosine phosphatase-1 B(PTP-1B)expression, and luciferase reporter assay was used to detect NF-kB activity. The results showed that treatment of HepG2 cells with TNF-α for 24 hours led to upregulation of PTP-1B and NF-kB activity in a dose-dependent manner. Inhibition of NF-kB by PI)TC significantly attenuated TNF-α-induced PTP-IB expression in HepG2 cells. Thus, the transactivation of NF-kB seems to play an important role in the expression of PTP-1B in HepG2 cells induced by TNF-α.