A novel nanometer non-viral gene transfer system for cartilage defects
10.3760/cma.j.issn.1001-8050.2009.05.181
- VernacularTitle:新型纳米非病毒基因转运系统治疗软骨缺损的实验研究
- Author:
Rui WANG
;
Ting GUO
;
Xiaofeng ZENG
;
Liwu ZHOU
;
Lei DONG
;
Junfeng ZHANG
;
Jianning ZHAO
- Publication Type:Journal Article
- Keywords:
Cartilage,articular;
Wounds and injuries;
Gene;
Nanometer
- From:
Chinese Journal of Trauma
2009;25(6):563-568
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a clinically applicable approach to enhance repair of cartilage defects by constructing an in vivo non-viral gene transfer system targeting chondrocytes. Methods High molecular weight chitosan (HMWC) was degraded to produce low molecular weight chitosan (LM-WC) that was combined with transforming growth factor-β1 (TGF-β1) plasmid to form stable nano-sizc complexes. After being tested in vitro firstly, these nano-size complexes were injected into the knee joint of New Zealand white rabbit models with full-thickness cartilage defects to detect their feasibility of delive-ring the growth factor gent in vivo. Results The results showed that LMWC/DNA nano-sizc comple-xes could deliver the gone into the cultured chondroeytes and cartilage tissue efficiently in vitro. When used in vivo, LMWC/TGF-β1 gene nano-size complexes could enhance the transfection efficiency and prolong the expression of TGF-β1 gone. In the animal models of articular osteechondral defect of rabbits, better healing and gentler degeneration could be observed in comparison with the control. Conclusion In vivo transfection of LMWC/TGF-β1 nano-size complexe is a safe and effective method to early promote the repair of osteochondral defects.
