Effects of ultrasound irradiating hydroxycamptothecin-loaded microbubbles on apoptosis and expression of related protein in human hepatoma SMMC-7721 cells
10.3760/cma.j.issn.1004-4477.2009.06.024
- VernacularTitle:超声辐照载羟基喜树碱微泡对肝癌SMMC-7721 细胞凋亡及相关蛋白表达的影响
- Author:
Ting ZHU
;
Tong SUN
;
Zhigang WANG
;
Pan LI
;
Sijing YAN
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Liver neoplasms,experimental;
Camptothecin;
Apoptosis
- From:
Chinese Journal of Ultrasonography
2009;18(6):529-532
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ultrasound irradiating hydroxycamptothecin (HCPT)-loaded microbubbles on apoptosis in human hepatoma SMMC-7721 cells, and to clarify its probable mechanism. Methods The SMMC-7721 cells were randomly divided into six groups as follows: control group, blank liposome microbubbles plus ultrasound, HCPT-loaded microbubbles, HCPT, HCPT plus ultrasound,and HCPT-loaded microbubbles plus ultrasound. Apoptosis was ascertained by Annexin V-Fire/ PI and TUNEL methods. The expression of Bcl-2 and Bax protein was detected by immunocytochemistry staining technique. Results In the group of HCPT-loaded microbubbles plus ultrasound,the apoptosis rate at early term was found to reach (12.18 ± 1.38)% by Annexin V-Fitc/PI assay, brown-colored positive apoptotic cells were observed and the apoptosis rate at advanced term was found to reach (34.25 ± 1.83)% with TUNEL method. The expression of Bcl-2 was found to decrease,the expression of Bax increase,and the ratio of Bcl-2/bax significantly decrease by immunocytochemistry staining technique in the same group. Differences with other groups were significant. Conclusions The method of ultrasound irradiating HCPT-loaded microbubbles can notability induce human hepatoma SMMC-7721 cells apoptosis. The decrease in the ratio of Bcl-2 to Bax might be responsible for cells apoptosis.
