A novel mechanism of hepatitis B virus mutation in hepatitis B e antigen negative chronic hepatitis B infection
10.3760/cma.j.issn.1000-6680.2009.06.008
- VernacularTitle:一种新的HBeAg阴性慢性乙型肝炎病毒变异机制
- Author:
Hongzhi XU
;
Jianlin REN
;
Qianguo MAO
;
Meiya CHEN
;
Fei ZHOU
;
Zhiping ZHANG
;
Yapi LU
;
Jinshui PAN
;
Jiayan CAI
;
Jing DONG
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Gene deletion;
Mutation;
Promoter regions(genetics);
Hepatitis B e antigens
- From:
Chinese Journal of Infectious Diseases
2009;27(6):352-356
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate mutation patterns in core promoter(CP)region of hepatitis B virus(HBV).Methods HBV DNA was extracted from sera of patients with chronic HBV infection.The CP sequence was amplified by polymerase chain reaction(PCR)and cloned into pMD19 T vector.The positive clones were then sequenced.The sequences were compared with known HBV genome in GenBank to identify the mutation sites and patterns of patients with chronic HBV infection.Results There were 74 clones from 21 patients with chronic HBV infection which were sequenced.The sequence comparisons showed that there was a 234-nucleotide deletion in CP region of HBV genome in 54 clones and a 245-nucleotide deletion in one clone.These deletion regions included CP,HBeAg initiation codon and direct repeat sequence(DR)Ⅰ regions,which named CP deletion(CPD).A1585T replacement mutation was also found in HBV strain with CPD,which indicated that there was linkage between these two mutations.Conclusions A novel mechanism of HBeAg negative chronic hepatitis B is observed,which includes deletions of CP and HBeAg initiation codon.Meanwhile,a simple and useful PCR method is developed to detect CPD.
