Adipophilin increases lipid accumulation in human vascular smooth muscle cells
10.3760/cma.j.issn.1000-6699.2009.03.021
- VernacularTitle:亲脂素增加血管平滑肌细胞的脂质集聚
- Author:
Fengling CHEN
;
Zhihong YANG
;
Xuanchun WANG
;
Yu LIU
;
Wenbai ZHOU
;
Xiaoyan SONG
;
Ye SHEN
;
Bo MOU
;
Renming HU
- Publication Type:Journal Article
- Keywords:
Adipophilin;
Human vascular smooth muscle ceils (HVSMCs);
Acetylated low-density lipoprotein(AcLDL);
Lipid accumulation
- From:
Chinese Journal of Endocrinology and Metabolism
2009;25(3):308-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of acetylated low-density lipoprotein (AcLDL) on the expression of adipophilin and the effect of adipophilin on AcLDL uptake and lipid accumulation in human vascular smooth muscle cells (HVSMCs)in order to approach the role played by adipophilin in genesis of macroangiopathy. Methodse HVSMCs were treated with various amount of AcLDL. Adipophilin expression levels were detected by Northern blot and Western blot. The effects of adipophilin on AcLDL uptake and lipid accumulation in HVSMCs were observed by the methods of siRNA, flow cytometry, enzymatic method and oil red stain. Results AcLDL dose-dependently increased adipophilin expression in HVSMCs. Silence adipophilin by siRNA decreased AcLDL uptake (decreasing by 38.7%, P<0. 05) and lipids accumulation (tfiglyceride and total cholesterol decreasing by 30.6% and 29.8% respectively, both P<0. 01) in HVSMCs, Conclusion Adipophilin is able to increase AcLDL uptake and lipid accumulation in HVSMCs, suggesting that it might play a role in enhancing atherosclerosis.
