Expression of smad2/3 protein and the effects of flunarizine on its expression in brain tissue following transient cerebral ischemic reperfusion in gerbils
- VernacularTitle:SMAD2/3蛋白在沙鼠脑缺血再灌注及氟桂利嗪干预后脑内表达的研究
- Author:
Jinhua FU
;
Zhiping HU
;
Huirong ZHENG
;
Yuantao HUANG
- Publication Type:Journal Article
- Keywords:
DNA-binding proteins/ME;
Brain isehemia/ME/DT;
Reporfusion injury/PC;
Flunarizine/PD[2] Wang Y,Chong CF,Morales M,et al. Protective effects of glial cell line-derived neurotrophic factor in ischemic brain injury Ann N Y Acad Sci,2002,962:423-437
- From:
Journal of Chinese Physician
2009;11(6):737-739
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of smad2/3 protein and the effects of flunarizine on the its expression in braln tissue following transient cerebral ischemie reperfusion in gerbils. Methods A cerebral ischemia-reperfusion model in gerbils was established by clamping both common carotids. Thirty-five gerbils were randomly divided into three groups, sham operation group, cerebral ischemia-reperfusion group and flunarizine treatment group. The expression of smad2/3 protein in brain tissue was detected by immunohistochemistry technique. Results Experimental results revealed that smad2/3 protein was expressed in neuroeyte in 35 gerbil brain. Compared with sham operation group, the expression of smad2/3 protein in neurecytes of cerebral isehemia-reperfusion group was evidently increased at the lst day, 3rd day and 7th day (P <0. 01). Compared with cerebral ischemia-reperfusion group, the expression of smad2/3 protein in neurecytes of gerbils in flunarizine treatment group was evidently decreased at these time point (P < 0.05). Condusions Smad2/3 protein was expressed in nettrcvytes of gerbils. Expression of smad2/3 protein in neuroeytes of gerbils was evidently increased following cerebral ischemic reperfusion, and its expression in flunarizine treatment group was evidently decreased.
