Reversal of multidrug resistance of K562/ADM cells in vitro by the monomer of traditional Chinese medicine extract and investigation of its molecular mechanism

VernacularTitle:中药提取物逆转K562/ADM细胞的耐药作用及其分子机制的探讨
Author: Gaojuan QIAO ; Guihai LI ; Weihua YANG ; Peie WEN ; Xia REN ; Hua FAN ; Tianhua TANG ; Haiquan REN ; Guosheng JIANG
Publication Type:Journal Article
Keywords: Scutellaria baicalensis; Gardenia jasminoides; P-glycoprotein; Leukemia
From: Journal of International Oncology 2009;36(6):474-476
CountryChina
Language:Chinese
Abstract: Objective To investigate the reversal effect of the monomer of traditional Chinese medicine on muhidrug resistance(MDR) and its possible mechanism in K562/ADM cell line in vitro. Methods With different concentrations of baicalin, geniposide administered to K562/ADM cells, the proliferation of K562/ ADM cells was detected by the MTY assay. Expression of mdr-1 mRNA, Topo Ⅱ mRNA was measured by semi-quantitive RT-PCR. Results Thatbaicalin and geniposide could increase the sensitivity of K562/ADM cells to adriamycin, multiples of reversion were 1.95 times and 1.46 times. The proliferation of K562/ADM cells was in-hibited obviously by baicalin and geniposide, the level of mdr-1 mRNA expression was down-regulated and the Topo Ⅱ mRNA was up-regulated(P<0.01 ). Conclusion Baicalin and geniposide may reverse the multi-drag-resistance of K562/ADM cells, which was related to the down-regulation of mdr-1 expression and up-reg-ulation of Topo Ⅱ beta expression.