Expressions of Ton-like receptor 9 protein in peripheral B and T lymphocytes from patients with systemic lupus erythematosus and their relationship with clinical parameters
10.3760/cma.j.issn.0412-4030.2009.07.006
- VernacularTitle:SLE患者外周血T、B淋巴细胞TLR9蛋白表达及与临床指标的关系
- Author:
Ou WU
;
Guoping CHEN
;
Hong CHEN
;
Xiangpei LI
;
Jun SHENG
;
Shushan ZHAO
;
Jiajia LI
;
Dongqing YE
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
T-lymphocyte;
B-lymphocyte;
Toll-like receptor 9
- From:
Chinese Journal of Dermatology
2009;42(7):457-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expressions of Toll-like receptor 9 protein (TLR9) in peripheral B and T lymphocytes in newly diagnosed, untreated patients with systemic lupus erythematosus (SLE) and their relationship with clinical parameters. Methods Blood samples were obtained from 35 newly diag-nosed, untreated patients with SLE and 16 healthy human controls. B, T lymphocytes and TLR9 protein were labeled with fluorescent antibodies, and the expressions of TLR9 protein were detected by flow cytometry in peripheral B and T lymphocytes. The relationship between TLR9 expression and clinical parameters was assessed. Results The proportions of B and T lymphocytes expressing TLR9 in newly diagnosed, untreated patients were (53.94±17.95)% and (49.33 ± 23.30)%, respectively, compared to (29.40 ± 10.54)% and (29.18 ± 14.78)%, respectively, in healthy controls (t = 6.11,3.73, respectively, both P < 0.01). Additionally,the proportion of B lymphocytes expressing TLR9 correlated negatively with SLE disease activity index (SLEDAI)(r = -0.39, P < 0.05), but positively with the level of serum IgA antibody (r = 0.74, P < 0.01).Condnsions The expression of TLR9 is elevated in peripheral T and B lymphocytes from patients with newly diagnosed, untreated SLE, and the proportion of TLR9-expressing B lymphocytes negatively correlates with SLEDAI, but positively correlates with the serum level of IgA antibody.
