Detection of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo
10.3760/cma.j.issn.0412-4030.2009.07.007
- VernacularTitle:白癜风患者外周血CD4+CD25+调节性T细胞的检测
- Author:
Minghui BAI
;
Jing WANG
;
Caixia TU
;
Yunying ZHANG
;
Min LIU
;
Guoyan LI
;
Liangrui ZHONG
- Publication Type:Journal Article
- Keywords:
Vitiligo;
T-lymphocytes,regulatory;
CD4-positive T-lymphocytes
- From:
Chinese Journal of Dermatology
2009;42(7):460-462
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the level of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4+ and CD4+CD25+ T lymphocytes in these samples. Results Compared with the controls, the percentage of CD4+CD25+ regulatory T lymphoeytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human con-trois [(2.43±0.30)% vs (3.49±0.39)% and (3.34±0.24)%, both P <0.05], but no significant difference was found between patients with stable vitiligo and normal human controls (P＞0.05). A significantly nega-tive correlation was observed between the percentage of CD4+CD25+ regulatory T lymphocytes and lesion area in patients with progressive vitiligo (r = -0.48, P <0.05), but not in patients with stable vitiligo (P ＞0.05). There was no significant correlation between the course of disease and the percentage of peripheral CD4+CD25+ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo (both P ＞ 0.05). Conclusion There is an abnormal proportion of peripheral CD4+CD25+ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.
