Effect of methylprednisolone pre-intervention on expressions of heat shock protein 27 and tumor necrosis factor alpha in spinal cord cells following ischemia-reperfusion injury in rats
10.3760/cma.j.issn.1671-7600.2009.06.014
- VernacularTitle:甲泼尼龙预干预脊髓缺血再灌注损伤对热休克蛋白27和肿瘤坏死因子α表达的影响
- Author:
Lei XIA
;
Tielin YIN
;
Lin DU
;
Ruonan ZHOU
;
Yisheng WANG
- Publication Type:Journal Article
- Keywords:
Methylprednisolone;
Spinal cord ischemia;
Reperfusion injury;
Heat-shock pro-teins;
Tumor necrosis factor-α
- From:
Chinese Journal of Orthopaedic Trauma
2009;11(6):555-558
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of methylprednisolone (MP) pre-intervention on ex-pressions of heat shock protein 27 (HSP27) and tumor necrosis factor alpha (TNF-α) in cells in rat spinal cord following ischemia-reperfusion injury. Methods One hundred and fifty male Sprague-Dawley (SD) rats were randomly divided into 3 equal groups: group A (control) in which the abdominal aorta was exposed without any treatment, group B in which the abdominal aorta was clipped for 30 minutes before reperfusion for 3 bours to establish a model of ischemia- reperfusion injury, and group C in which intravenous MP injection was conducted 30 minutes before the establishment of the ischemia-reperfusion injury model. Three hours later the spinal cords were harvested. Pathological changes of spinal cord cells were observed with HE staining and expressions of HSP27 and TNF-α in spinal cord cells were observed with immunohistochemical staining. The motor function of hind-limbs before was evaluated before sample harvest. The data were analyzed with SPSS software. Results There were significant differences between groups A and B in the expressions of TNF-α and HSP27. Compared with group B, the expression of TNF-α decreased and HSP27 increased in group C, with statistically significant differences between the 2 groups. The motor function score of hind-limbs decreased in group B but improved in group C. Conclusions Since MP can decrease the expression of TNF-α and up-regulate the expression of HSP27, it has a potency of neuro-protection. Spinal cord ischemia-reperfusion injury can be avoided or decreased after MP pre-intervention.
