Comparison of three methods: Giemsa, Paracheck, Polymerase chain reaction in detection of malaria parasite at Daksong commune, Krongchro district, Gialai province, June 2002
- Author:
Nguyen Van Tuan
- Publication Type:Journal Article
- Keywords:
Giemsa, Paracheck, Polymerase chain reaction, malaria
- MeSH:
Malaria;
Polymerase Chain Reaction;
Parasites;
diagnosis
- From:Journal of Malaria and parasite diseases Control
2003;3():55-63
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Using both three methods of Giemsa, Paracheck and Polimerase chain reaction (PCR) determined P.falciparum in P. falciparum malaria children patients under 10 years old after 14 days dosed with antimalarial drugs and healthy Bana minority people in Daksong commune, Krongchro district, Gialai province in June 2002. The sensitivity and specificity of Paracheck were found to be 91.37%, 86.1% respectively as compared to Giemsa method and 77.3% and 97.7% as compared to PCR. The sensitivity and specificity of Giemsa method was 77.3% and 100% respectively. By using PCR, 3 of 25 samples negative with Giemsa and 20 samples negative with Giemsa Paracheck were detected to be positive at the day 14 after administration with Fansidar or artesunate