Polymorphism of IL-10 and Helicobacter pylori infection on gastric cancer genesis
10.3760/cma.j.issn.1007-631X.2009.07.020
- VernacularTitle:白细胞介素10基因多态性及幽门螺杆菌感染与胃癌的相关性研究
- Author:
Kaihua ZHU
;
Yumin LI
;
Lingling LIU
;
Dan ZHANG
;
Xiaofan TANG
;
Jun ZHAO
;
Wenting HE
;
Tao LIU
;
Yuxin SONG
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Iterleukin-10;
Polymorphism,single nucleotide;
Helicobacter pylori;
Immunoblotting
- From:
Chinese Journal of General Surgery
2009;24(7):577-581
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the relationship between 1082 site A/G polymorphism in interleukin-10 gene and different virulence factors of Helieobacter pylori in Gansu Province and susceptibility to gastric cancer genesis. Methods Polymerase chain restriction fragment length polymorphism and direct sequencing were performed to analyze the genotype of the A/G polymorphism in its-1082 site of promoter region, and immunoblotting was performed to test different virulence factors antibody of H. pylori. Results respectively in the gastric precancerous lesion group. The frequency of AG + GG genotype was statistically higher in the gastric precancerous lesion group compared to the control group (P =0. 018), the risk gastric frequency of AA, AG, GG genotypes were 58.4% ,35.8% ,5.8%, respectively in the gastric cancer group. The frequency of AG + GG genotype was higher in the gastric cancer group compared to the control group (P =0. 010), moreover, individuals with the IL-10-1082AG + GG genotype rose to 2. 31 fold risk for gastric there was an increased risk of gastric precancerous lesion and developing gastric cancer for those carrying both AG + GG genotype and seropositive H. pylori I, with 9.73 fold risk. Conclusion There was a relationship between IL-10-1082 A/G polymorphism and susceptibility to gastric cancer.
