Fluorescence in situ hybridization for detection of bladder cancer
10.3760/cma.j.issn.1673-4203.2009.07.020
- VernacularTitle:荧光原位杂交在膀胱癌诊断中的应用研究进展
- Author:
Yu DU
;
Linlin MA
;
Ye TIAN
- Publication Type:Journal Article
- Keywords:
bladder eaneer;
fluorescence/n situ hybridization;
uroVysion;
urine cytology
- From:
International Journal of Surgery
2009;36(7):488-491
- CountryChina
- Language:Chinese
-
Abstract:
Fluorescence in situ hybridization (FISH) is a kind of technique that uses fluoreseently la-beled DNA probes to assess cells for genetic alterations. UroVysion is a technique that uses FISH to detect bladder cancer in the voided urine by four fluorescently labeled DNA probes to the pericentromeric regions of chromosomes 3 ( CEP3 ) ,7 (CEPT), and 17 (CEP17) and to the 9p21 locus ( LSI 9p21 ) location of the p16 tumor suppressor gene. It is reported that sensitivity of FISH was higher than cytology for the detection of all stages and grades of bladder cancer. FDA has approved UroVysion for the detection of recurrent bladder cancer in voided urine specimens from patients with a history of bladder cancer in the year 2001 and from pa-tients with gross or microscopic hematuria but no previous history of bladder cancer in the year 2005. Fur-thermore, UroVysion can benefit the assessment of bladder cancer patients receiving BCG treatment.
