Research actuality of chemosensitivity testing in chemotherapy
10.3760/cma.j.issn.1673-4203.2009.08.024
- VernacularTitle:化疗药敏试验的研究现状
- Author:
Ning ZHAO
;
Xiang QU
;
Zhongtao ZHANG
- Publication Type:Journal Article
- Keywords:
chemosensitivity test;
tumor;
chemotherapy
- From:
International Journal of Surgery
2009;36(8):565-568
- CountryChina
- Language:Chinese
-
Abstract:
This paper explains the research status of chemotherapy sensitivity test in three aspects of history of development, specific methods and clinical application. Chemotherapy sensitivity test is an important way to achieve individual treatment of cancer, after more than 60 years, there are two major categories(in vivo method and in vitro method) of more than 10 kinds of methods. The basic steps of sensitivity test inelude culture of primary tumor cells, chemotherapy drugs mixed, the reaction mixture, observing the results of detection and analysis of indicators. This paper focuses on basic principles, main steps and characteristics of six methods, such as the renal capsule of nude mice model of human cancer, the difference between staining cell, tetrazolium salt colorimetric, adenosine triphosphate based bioluminescence tumor chemosensitivity assay, collagen gel embedded culture method and targeting molecule sensitivity assay. Through the results of several clinical trials, it can be seen that chemotherapy under the guidance of drug sensitivity test significantly improved more than experience chemotherapy in efficient rate, median progression-free survival time, survival time, clinical complete remission rate, pathological complete remission rate, etc.
