Content Determination of Pei-bao Capsule by HPLC
10.3760/cma.j.issn.1673-4246.2009.04.044
- VernacularTitle:HPLC测定胚宝胶囊的含量
- Author:
Xinlan YU
;
Tuoyun GUO
;
Ge LI
;
Zhangzhao JIN
- Publication Type:Journal Article
- Keywords:
Pei-bao capsule;
HPLC;
Lysine hydrochloride
- From:
International Journal of Traditional Chinese Medicine
2009;31(4):364-365
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for quality control of Pei-bao capsule. Methods The content of lysine derivatized from 2,4-dinitoflruorobenzene was determined by HPLC, with chromatographic column being the agilent Zorbax SB-C18 column (250 mm×4.6 mm, 5 μm) , mobile phase being acetonirile-0.04mol/L, potassium dihydrogen phosphate (40 : 60), the flow rate being 1.0 ml/min, the column temperature being 30 ℃, and wavelength being 360 nm. Results It showed good linear coorelation when the concentration of Lysine hydrochloride being within 0.39～ 19.3μg/ml (γ =0.9999) , and the average sample recovery rate was 100.2%. Conclusion This method is simple, accurate, stable and reliable. It can be used for quality control of Pei-bao capsule.
