Immunohistochemistry study on the infectivity of hepatitis B viruses on human hepatic stellate cells
10.3760/cma.j.issn.1673-4203.2009.07.007
- VernacularTitle:免疫组化方法观察乙肝病毒感染肝星状细胞的研究
- Author:
Xuemei MA
;
Yu WANG
;
Hongying ZHAO
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
hepatic stollate cell;
infection;
replication
- From:
International Journal of Surgery
2009;36(7):448-451
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to observe whether HBV can infect HSC in vitro. LX-2 cells, the human activated HSC cell line, were incubated with human serum containing HBVDNA at final concen-trations from 0.01 to 10 copies/cell and harvested after 24,48 and 72 hours. Hepatitis B surface antigen (HBsAg) and hepatitis B core antigen(HBcAg) were detected by lmmunohistochemistry dyed by DAB. By Immunohistochemistry, no positive particles in LX-2 cells, a few of dark brown particles (HBsAg and HB-cAg) were found in cytoplasm and nucleoli of HepG2.2. 15 cells,a lot of clark brown particles were found in liver biopsies of patients with chronic hepatitis B. In vitro, there was no evidence that HBV can infect and express antigens in LX-2 cells.
