The regulating role of TNF-α with dexamethasone and shenfu separate and joint administration of the flap after ischemia-reperfusion injury
10.3760/cma.j.issn.1008-6706.2009.07.016
- VernacularTitle:地塞米松与参附对大鼠皮瓣缺血再灌注损伤后肿瘤坏死因子α的调节作用
- Author:
Wei HUANG
;
Xiaomin LAI
;
Yingpeng ZHANG
;
Bo ZHONG
;
Suwei WANG
- Publication Type:Journal Article
- Keywords:
Reinfusion injury of ischemic;
TNF-α;
Sbengfu injection;
Dexamethasone
- From:
Chinese Journal of Primary Medicine and Pharmacy
2009;16(7):1178-1179
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the protective effects and mechanisms of dexamethasone and Shenfu sepa-rate and joint administration of flap after ischemia-reperfusion injury. Methods 40-month-old fairly healthy rats were randomly divided into 4 group as A, B, C, D, and to product the abdominal island flap, then blocking the flow of blood of the pedicle artery respectively before 30 minutes when injecting with normal saline (1 ml/kg), dexametha-sone (1 ml/kg), Senate (10 ml/kg), joint injection with dexamethasone (1 ml/kg) and Shenfu (10 ml/kg). 4 groups of animals' blood samples were collected from the pedicle vein before the time of I hour when blocking vascu-lar pedicle and reperfusion after the time of 1 h, 6 h, 12 h, 24 h, respectively. Then the plasma concentration of TNF-α was measured. Results The concentration of TNF-α in the treatment group was significantly lower than the blank group(P <0.01). And the group D and the group B、C has significant differences either(P <0.01). Conclu-sion Using Dexamethasone, Shenfu injection in early can reduce the concentration of TNFα in repeffusion injury of flap and has a protective effect on the flap, but make better effect in combined.
