Effects of propofol on expression of death-associated protein ktnase mRNA in a rat model of traumatic brain injury
10.3760/cma.j.issn 0254-1416.2009.07.021
- VernacularTitle:异丙酚对大鼠创伤性脑损伤时DAPK mRNA表达的影响
- Author:
Min LIANG
;
Yan LI
;
Yutian WANG
- Publication Type:Journal Article
- Keywords:
Propofol;
Brain injuries;
Apoptosis;
Neurons;
Protein-serine-threonine kinases
- From:
Chinese Journal of Anesthesiology
2009;29(7):652-654
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of propofol on death-associated protein kinase (DAPK) mRNA expression in a rat model of traumatic brain injury (TBI). MethodsSixty male Wistar rats with a mean age of 3-4 months and mean body weight of 250-300 g were randomly divided into 6 groups ( n = 10 each) : Ⅰ normal control;Ⅱ sham operation; Ⅲ TBI; Ⅳ normal saline (NS) +TBI;Ⅴ fat emulsinn+ TBI and Ⅵ propefol + TBI. TBI was produced according to Feeney et al. In group Ⅲ-Ⅵ the animals were killed and their brains were removed at 24 h after brain contusion. NS 2 ml·kg-1 · h-1 , 10% fat emulsion 2 ml · kg-1 · h-1 and propofol 2 ml·kg-1·h-1 were infused iv for 4 h after lead trauma in group Ⅳ, Ⅴ and Ⅵ respectively. DAPK mRNA expression in the brain tissue was determined by RT-PCR and neuronal apeptosis was assessed by TUNEL. Results The expression of DAPK mRNA and neuronal apoptosis were significantly increased in the TBI group as compared with normal control and sham operation groups. Propefol infusion significantly attenuated neuronal apoptosis and reduced DAPK mRNA expression as compared with TBI, NS and FE groups. ConclusionPropofol can protect the brain from traumatic injury by suppression of the expression of DAPK mRNA.
