Relationship of proliferation and activation of T lymphocyte subsets and disease progression in human immunodeficiency virus-I-infected individuals
10.3760/cma.j.issn.1000-6680.2009.07.008
- VernacularTitle:人类免疫缺陷病毒感染者T淋巴细胞亚群增殖、活化与疾病进展的相关性
- Author:
Zhenhuan CAO
;
Haiying LI
;
Lina MA
;
Qiaoli PENG
;
Yakun TIAN
;
Lingxian SHI
;
Yi JIN
;
Zhimin HE
;
Nan GENG
;
Xinyue CHEN
- Publication Type:Journal Article
- Keywords:
HIV-1;
Acquired immunodeficiency syndrome;
T-lymphocyte subsets Celldivision Lymphocyte activation
- From:
Chinese Journal of Infectious Diseases
2009;27(7):418-422
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship of proliferation and activation of T lymphocyte subsets and disease progression in antiretroviral-naive human immunodeficiency virus(HIV)-1-infected individuals.Methods Forty-nine antiretroviral-naive,chronically HIV-1 infected patients and 16 healthy,HIV-1 negative controls were enrolled in this study.The patients were divided into 3 groups according to their CD4+T cell counts:<200×106/L,(200-350)×106/L and>350×106/L.Peripheral blood mononuclear cells(PBMC)were isolated.T cell proliferation index was measured by Ki-67 staining.T cell activation was detected by CD38 staining.The samples were analyzed by flow cytometry.The data were compared by one-way ANOVA.Results The percentage of Ki-67+cells in CIM+T ceils was 7.92%±4.37%in CD4+T cell<200×106/L group,which was significantly higher than those 0.39%d:0.24%in control group,2.61%±2.12%in(200-350)×106/k group and 2.65%±2.13%in>350 X106/L group(F=21.961,P<0.01).The percentage of Ki-67+cells in CD8+T ceils in CD4+T cells<200×106/L group was 2.87%±1.13%,which was also much higher than those in other 3 groups(0.15%±0.90%,1.40%±1.17%,1.22%±0.80%,respectively F=19.203,P<0.01).The Ki-67'CD4'T cells and Ki-67+CD8+T cells were inversely correlated with CD4+T cell counts(r=-0.654,r=-0.539,respectively;P350×106/L group(F=14.333,F=15.412,respectively;P<0.01).The expressions of Ki-67+ on CD4+ and CD8+T cells were positively correlated with CD38 expression(r=0.527,r=0.391,respectively;P=0.002).Conclusions The proliferation and activation of T lymphoeytes are enhanced during HIV/AIDS disease progression.And T eell activation may be the result of persistent immune activation.
