The mechanism study of Chinese traditional medicine mixture on protection of vascular endothelial cell from apoptosis in cerebral ischemia-reperfusion injury
- VernacularTitle:中药脑泰方保护脑缺血再灌注损伤中血管内皮细胞凋亡机制探讨
- Author:
Hu TAN
;
Yi CHEN
;
Dingxiang LI
- Publication Type:Journal Article
- Keywords:
Ligustieum ehuanxiong/PD;
Lumbricus/PD;
Astragalus membranaceus/PD;
Endothelium,vascular/DE;
Brain ische-mia/ZD;
Reperfusion injury/PC;
Apoptosis
- From:
Journal of Chinese Physician
2009;11(7):892-895
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanisms of Chinese traditional medicine mixture protection of vascular endothelial cell from apoptosis in cerebral ischemia-reperfusion injury. Methods Healthy, clean SD rats were randomly divided into 4 groups: Sham opera-tion group (SOG), cerebral ischemia reperfusion injury group (IRG), cerebral ischemia preconditioning group (IPG) and Chinese tradi-tional medicine mixture preconditioning group (CPG). Furthermore, IRG, IPG and NPC were divided into 4 sub-groups: 1 d, 7d, 14d, 21d subgroup, according to the different time point since ischemia-reperfusion took place. And in CPG, Naotai formula extract was used. Cere-bral vascular endothelial cells of rats were removed and Hoechst 33258 staining and the DNA gradient bands were used to detect the apoptosis of these cells. Then the influence of Naotai formula extract on caspase-3, 8 and 9 activation, and Bid lysis was examined by Western-blot, and the mechanisms of Chinese traditional medicine mixture protection of vascular endothelial cell were investigated from apoptosis signal pathway. Result Naotai formula extract can inhibit the apoptosis of endothelial cells in ischemia-reperfusion injury, and it also can inhibit the activation of caspase-3, 8 and 9, thus inhibit the lysis of Bid into tBid. Conclusion Naomi formula extract inhibit the apoptosis of endo-thelial ceils in ischemia-reperfusion injury via apoptosis signal pathway.
