Effect of intrathecal administration of a mixture of butorphanol and ketamine on cAMP-PKA-CREB signal transduction pathway in spinal dorsal horn of rats with inflammatory pain
10.3760/cma.j.issn.0254-1416.2009.08.010
- VernacularTitle:鞘内注射布托啡诺混合氯胺酮对炎性痛大鼠脊髓背角cAMP-PKA-CREB信号转导通路的影响
- Author:
Yueling WANG
;
Wenjuan MA
;
Yong YANG
;
Qulian GUO
- Publication Type:Journal Article
- Keywords:
Butorphanol;
Ketamine;
Cyclic AMP;
Cyclic AMP-dependent protein kinnses;
Cyclic AMP response element-binding protein;
Pain;
Injections,spinal
- From:
Chinese Journal of Anesthesiology
2009;29(8):712-715
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of intrathecal administration of a mixture of butorphanol and ketamine on cAMP-PKA-CREB signal transductian pathway in the spinal dorsal ham of the rats with inflammatory pain. Methods Twenty-four male SD rats, weighing 240-280 g,in which intrathecal catheters were successfully placed, were divided into 4 groups randomly (n = 6 each): inflammatory pain group (group IP), butorphanol group (group B), ketamine group (group K), and butorphanol + ketamine group (group BK). The inflammatory pain was induced by injection of 5% formalin 50 μl into the plantar surface of left hind paw. Normal saline 10 μl, butorphannl 12.5 μg, ketamine 50 μg, and a mixture of butorphanol 12.5 μg and ketamine 50 μg was injected intrathecally 30 min before subcutaneous injection of formalin in group IP, B, K and BK respectively.Pain intensity score (PIS) was used to assess pain behavior every 5 min within an hour after subcutaneous injection of formalin. The animals were killed at 2 h after subcutaneous injection of formalin, and the L5 segment of the spinal cord was removed for determination of protein kinase A (PKA) and phosphorylated cAMP response element binding protein (p-CREB) expression using immunohistochemistry. Results Fonnahn administration induced pain behaviour expressed as two phases. PIS scores, PKA and p-CBEB expression, and staining scores were significantly lower during the fast and second phases in group BK than in group IP (P < 0.05 or 0.01), while no significant differences were found in the indices mentioned above between group B and IP and between group K and IP (P>0.05). Conclusion lntrathecal injection of a mixture of butorphanol and ketamine can reduce inflammatory pain in rats, and the mechanism may be related to the cAMP-PKA-CREB signal transduction pathway.
