Analysis of the determination of NO and NOS in brain and myocardium of depression model rats
- VernacularTitle:抑郁模型大鼠脑和心肌组织中一氧化氮一氧化氮合酶水平测定分析
- Author:
Zhengjun XIAO
;
Yiming WANG
- Publication Type:Journal Article
- Keywords:
Depression;
Brain;
Myocardium;
Nitric oxide;
Nitric oxide synthase
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2009;18(7):589-590
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study nitric oxide (NO) and nitric oxide synthase (NOS) in brain and myocar-dium of depression model rats and to explore the mechanism of brain and myocardium injuryed. Methods The de-pression model rats were produced by chronic mild unpredictable stress and separation. The behavior of rats were detected by open field test and sucrose consumption test. The contents of NO and NOS were determined with spec-trophotometric method. Results Compared with the normal control, the contents of NO [Brain ( 8.97±2.22 )μmol/g prot vs ( 1.86±1.28 )μmol/g prot; Myocardium (9.67±1.53) μmol/g prot vs (2.67±1.08)μmol/g prot] and NOS[Brain(9. 50±1.89) U/mg prot vs (2.31±0. 97) U/mg prot; Myocardium( 11.20±1.47) U/mg prot vs(2.53±0.97)U/mg prot] in brain and myocardium were significantly increased (P<0.01)of depression model rats. Conclusion The contents of NO and NOS increase significantly in brain and myocardi-um of depression model rats and it may induce the injury on brain and myecardium of them.
