Expression of S-phase kinase associated protein 2 in liver tissues of rats with acute liver failure
10.3760/cma.j.issn.1000-6680.2009.08.003
- VernacularTitle:S期激酶相关蛋白2在急性肝功能衰竭大鼠肝组织中的表达
- Author:
Zhejuan YANG
;
Yongping CHEN
;
Hailong LIN
- Publication Type:Journal Article
- Keywords:
S-phase kinase-associated proteins;
Liver failure,acute;
Microencapsulatedhepatocyte;
Liver transplantation;
Disease models,animal
- From:
Chinese Journal of Infectious Diseases
2009;27(8):461-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of S-phase kinase associated protein 2 (Skp2) in rats with acute liver failure (ALF) and its significance. Methods There were 256 male SD rats used in this study, among which 240 were injected with D-galactosamine (D-GaIN) to set up ALF model. The rats were divided into 3 groups: ALF model group, free hepatocellular transplantation group, microencapsulated hepatocyte transplantation group, which were intraperitoneally injected with 2 mL of RPMI 1640 culture medium, free hepatocellular suspension and microencapsulated hepatocyte suspension, respectively. The other 6 rats were in control group and the rest 10 rats were used for hepatocyte isolation. Expressions of Skp2 protein in hepatocytes of rats at different time points were detected by immunohistochemical technique. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBil) were detected by automatic biochemistry analyzer. The survival rate in each group was observed. Comparisons among groups were done using one-factor analysis of variance. Results Levels of ALT, AST and TBil decreased more significantly by intraperitoneal transplantation of microencapsulated hepatocytes than those by intraperitoneal transplantation of free hepatocytes (P<0. 05). Skp2 labeling indices after 36 h of injection in ALF model group, free hepatocellular transplantation group and microencapsulated hepatocyte transolantation grouo were (28. 2±6.1) %, (41.4± 10. 5) % and (68. 0±10.8) %, respectively (F=29. 08 , P<0. 05). There were 4, 6 and 11 out of 15 rats survived in the 3 groups, respectively. Conclusion The dynamic observation of Skp2 expression could be used to judge the regeneration of hepatocytes.
