Immunological regulation mechanism of epigallocatechin-3-gallate on collagen-induced arthritis

VernacularTitle:(一)一表没食子儿茶素没食子酸酯对胶原诱导性关节炎小鼠免疫调节机制研究
Author: Dongyi HE ; Hong NIE ; Yan WANG ; Xiaofang ZHANG ; Peiguo ZHENG ; Lieming XU
Publication Type:Journal Article
Keywords: Arthritis,rheumatoid; Arthritis,experimental; Mice,inbred DBA; Epigallocatechin3-gallate
From: Chinese Journal of Rheumatology 2009;13(8):538-541
CountryChina
Language:Chinese
Abstract: Objective The therapeutic effect of epigallocatechin-3-gallate (EGCG) on the collageninduced arthritis model (CIA) was observed and its immunological mechanism was analyzed. Methods EGCG was administered to CIA mice and PBS was admitted as negative control. The severity of CIA was evaluated by clinical scores and histopathological assessment (H-E staining). Immunological mechanisms inv-suppressive effect on IL-17 secretion of CD4+T cells (EGCG group: 0.41%; PBS group: 4.05% ) and inhibitive activity of C Ⅱ -reactive splenocytes proliferation. There was statistical significant difference between IKB expression and down-regulate phosphorylated IKB expression in lymph node cells of CIA mice.Conclusion EGCG can significantly ameliorate the severity of CIA. The therapeutic mechanisms may be related to inhibition of C Ⅱ -reactive splenocyte proliferation and IL-17 secretion and via inhibiting the activity of NF-κB by inducing the expression of IKB and by suppressing the expression of phosphorylated IKB in CIA mice.