Changes of T-lymphocyte function subsets and IL-4 and IFN-γ after irradiation injury
10.3760/cma.j.issn.0254-5098.2009.04.010
- VernacularTitle:辐射损伤后小鼠T细胞功能亚群及其IL-4和IFN-γ的变化
- Author:
Hui ZHENG
;
Rong ZHEN
;
Xumin TU
;
Zhixing ZHANG
- Publication Type:Journal Article
- Keywords:
Radiation;
Immune;
Harm;
T-lymphocyte subsets;
IL-4;
IFN-γ
- From:
Chinese Journal of Radiological Medicine and Protection
2009;29(4):386-388
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of T-lymphocyte function subsets,IL-4 and IFN-γ cell factor in different dose and stage after irradiation.Methoda The C57BL/6j mice were divided into shammed irradiation group and model groups.The radiation hurt model was induced by 60Co gamma rays(0.7,1.4,2.8 and 5.6 Gy).The changes of T-lymphocyte subsets CD3,CD4,CD8,IL-4 and IFN-γ in spleen cells were analyzed by flow cytometry in acute injury stage and recovery stage after irradiation.Results The lymphocyte subsets CD3 +,CD4+ and CD8 + decreased after irradiation,which were related to the irradiation dose.At 1 day after irradiation,the decreasing level of IFN-γ was higher than that of IL-4.When irradiation dose was over 2.8 Gy,IL-4 / IFN-γ showed a markedly increased compared with control group.At 25 days after irradiation,CD3 +,CD4+,CD8 +,CD4 +/CD8 +,IL-4 and IFN-γ recovered obviously,but they did not recover to the normal level of shammed irradiation group.Conclusions The depression of mouse immune function induced by γ-irradiation might be caused by changes of CD3,CD4,CD8 and CD4/CD8 ratio,especially the imbalance of IL4 and IFN-γ.
