Expression of CD95 on circulating endothelial cells in patients with hemorrhagic fever with renal syndrome
10.3760/cma.j.issn.1000-6680.2009.08.010
- VernacularTitle:肾综合征出血热患者循环内皮细胞CD95表达的临床研究
- Author:
Jianjun TIAN
;
Ying ZHANG
;
Jingyao ZHANG
;
Shuzhen CHANG
;
Zhongtao GAI
- Publication Type:Journal Article
- Keywords:
Hemorrhagic fever with renal syndrome;
Endothelial cells;
Antigens,CD95;
Antigens,CD141;
Antigens,Ed31
- From:
Chinese Journal of Infectious Diseases
2009;27(8):487-490
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic expressions and clinical significance of CD141, CD31 and CD95 on circulating endothelial cells (CEC) in febrile and polyuria phases of patients with hemorrhagic fever with renal syndrome (HFRS). Methods Expressions of CD141, CD31 and CD95 in the peripheral blood of patients with HFRS in febrile and polyuria phases were detected by flow cytometry. Comparisons among groups were done by one-factor analysis of variance. Results The percentages of CD141+ CD31+ cells in the peripheral blood cells from patients with HFRS in febrile and polyuria phases were 9.47% ±1.98 % and 8. 26% ±1.55 %, respectively, which were both higher than that (7.05%±1.45%) in healthy controls (F=8. 42; P=0. 000 and P=0. 029, respectively), and that in febrile phase was higher than that in polyuria phase (P = 0. 048). The mean fluorescent intensity (MFI) of CD95 on CEC of HFRS patients in febrile and polyuria phases were both significantly higher than that in healthy controls (F=19. 93; P=0. 000 and P=0. 000 respectively), and that in febrile phase was higher than that in polyuria phase (P=0. 049). In the febrile phase of HFRS,the MFI of CD95+ on CEC in patients with all clinical types were all higher than that in healthy controls (F= 17. 36; all P=0. 000), and that in severe (critical) type was the highest and higher than those in mild type and moderate type (P=0. 002 and P=0. 009, respectively). Conclusion The proportion of CEC and expression of CD95 on CEC are possibly related with the phase and severity of HFRS.
