Effect of dehydroepiandrosterone on thymocytes in vitro
10.3760/cma.j.issn.1007-7480.2009.08.011
- VernacularTitle:脱氢表雄酮在体外对胸腺细胞的调节机制研究
- Author:
Jun LIAGN
;
Lingyun SUN
- Publication Type:Journal Article
- Keywords:
Dehydroepiandrosterone;
Thymus gland;
Apoptosis;
Antigens,CD95
- From:
Chinese Journal of Rheumatology
2009;13(8):547-549
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of dehydroepiandrosterone (DHEA) on the immune system by measuring the apoptosis and expression of Fas/FasL mRNA of thymocytes. Methods The cell viability of thymocytes was determined by MTT assay. Thymocyte apoptosis was detected by agrese gel electrophoresis. The expression of Fas and Fas-L was analyzed by a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method. Results DHEA decreased the cell viability of thymocytes and induced thymocytes apoptosis and increased the levels of mRNA of Fas/FasL. Conclusion DHEA can induce apoptosis of thymocytes through Fas/Fas-L pathway. It suggests that DHEA may play a role in certain autoimmune diseases related to apoptosis disturbance.
