Study on the pit patterns of colorectal neoplastic lesions with the expression of PCNA and survivin mRNA
- VernacularTitle:PCNAmRNA和Survivin mRNA在不同大肠肿瘤性病变腺管开口组织中表达关系的研究
- Author:
Dingxin WANG
;
Liwei ZHANG
;
Jiansheng ZHANG
;
Guozhang XING
- Publication Type:Journal Article
- Keywords:
Proliferating cell nuclear antigen/BI;
Microtuble-associated proteins/BI;
Intestinal neoplasms/PA/ME
- From:
Journal of Chinese Physician
2009;11(7):882-884
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship of pit pattern in the progress of colorectal and the expression of PCNA and survivin in pit pattern. Methods Chromoendoscopy observation results were recorded according to the pit pattern classification proposed by Kudo. These results were divided into 6 groups according to the pit pattern and the results of histophathology. Expression of PCNA and surviving mRNA was detected by RT-PCR. Statistical analysis was performed using χ2 test , Fisher's exact test or Spearman rank correlation with SPSS 12.0 software package. Results In 6 groups classified according to the pit patterns, the expression rate of PCNA mRNA in type Ⅰ, Ⅱ, Ⅲ L, Ⅳ, V 1 and VN were 0% (0/13), 12.5% (1/8), 38.9% (7/18), 66.7% (12/18), 75.0% (3/4) and 100.0% (9/9), the expression rate of survivin mRNA in type Ⅰ,Ⅱ, Ⅲ L, Ⅳ, V 1 and V N were 0%, (0/13), 12.5% (1/8), 22.2% (4/18), 44.4% (8/18), 50.0% (2/4) and 77.8% (7/9), respectively. Conclusions The expression of survivin and PCNA mRNA had positive correlation in the coloree-tal lesions classified whether by the pit patterns or histophathology. Study of the two genes simultaneously could complement each other and reflect the biological characteristics of colorectal cancer more accurately.
