CXCR4 gene transfection enhancing the distribution of dMSCs to the wounded skin of rats with combined wound and irradiation injury
10.3760/cma.j.issn.0254-5098.2009.04.001
- VernacularTitle:CXCR4基因转染促进移植dMSCs向放创复合伤大鼠创面分布的研究
- Author:
Zhaowen ZONG
;
Tianmin CHENG
;
Xinze RAN
;
Yongping SU
;
Shiwu DONG
;
Nan LI
;
Junping WANG
;
Guoping AI
;
Zheng LI
- Publication Type:Journal Article
- Keywords:
CXCR4;
Gene transfection;
Dermal muhipotent stem cells;
Combined injury
- From:
Chinese Journal of Radiological Medicine and Protection
2009;29(4):351-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether the transplanted dermal multipotent stem cells(dMSCs)transfected by adenovirus vector of CXCR4(Adv-CXCR4)can distribute more frequently to the wound of rats with combined wound and irradiation injury.Methods dMSCs transfected by Adv-CXCR4(group A),or transfected by adenovirus vector of green fluorescent protein(group B),and non-transfected dMSCs were labeled with 3H-TdR and then transplanted into combine-injured rats.The amount of dMSCs in wound were determined by liquid scintillation,and wounds healing process was observed by measuring the remaining wound area.Results From the 5th day after transplantation,the amount of dMSCs in the wound of group A accounted for 1.95%-3.85% of the total transplanted dMSCs,significantly greater than those in group B and group C,which accounted for 1.07%-1.86% of the total transplanted dMSCs.The remaining wound area in group A was smaller than those in group B and group C from day 12 after injury,and the healing time of group A was 1.5 day ahead than group B and group C.Conclusions dMSCs transfected by Adv-CXCR4 distributes more frequently to the wound of combine-injured rats and could accelerate wound healing.
