Effects of multiple low dose radiation on the apoptosis of splenocytes and immune factor in male diabetic rats
10.3760/cma.j.issn.0254-5098.2009.04.004
- VernacularTitle:多次低剂量照射对雄性糖尿病大鼠脾细胞凋亡和免疫因子的影响
- Author:
Yanbo LI
;
Caixia GUO
;
Lihua DONG
;
Jianfeng WANG
;
Shuchun LIU
;
Zhe LU
;
Shouliang GONG
- Publication Type:Journal Article
- Keywords:
Low dose radiation;
Diabetes mellitus;
Spleen;
Apoptosis;
Immune factor
- From:
Chinese Journal of Radiological Medicine and Protection
2009;29(4):364-367
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of multiple low dose radiation(LDR)on the apoptosis of splenocytes and immune factors in diabetes mellitus(DM)rats.Methods The rats were randomly divided into control,DM and DM + LDR groups.The irradiation doses were 25,50 and 75 mGy,and the irradiated times were 15.At the fourth weekend after the DM rats irradiated,the apoptotie rate and TCRαβ percentage of splenoeytes were detected by flow cytometry,and the content of IL-2 in both serum and supernatant of cultured splenocytes were detected by ELISA.Results Compared with that in the control,the body weight(BW)decreased in the DM and DM + LDR groups,particularly in DM group.The blood glucose(BG)level in the DM + LDR groups was higher than that in the control,but decreased significantly as compared with that in the DM group(P < 0.01).As compared with those in the control,the apoptotic rate in DM + 50 mGy(P < 0.05)and the content of serum IL-2 in DM + 75 mGy group(P < 0.01)all increased significantly,while the content of IL-2 in supernatant of cultured splenocytes decreased significantly in the DM + LDR groups.Compared with those in the DM group,the apoptotic rate and the percentage of TCRαβ in splenocytes in the DM + LDR groups(P < 0.01-P < 0.001)and the content of IL-2 in serum in DM + 50 mGy group(P < 0.01)decreased significantly.Conclusions The multiple LDR could weaken the loss of BW and increase of BG caused by DM,decrease the splenocyte apoptosis induced by DM,and regulate the immune factors.
