Effect of Yiqi Huoxue Recipe on the expression of TGF-β1,TNF-α in rats
10.3760/cma.j.issn.0254-5098.2009.04.009
- VernacularTitle:益气活血中药对大鼠TGF-β1、TNF-α的影响
- Author:
Hai ZHANG
;
Bingsheng WANG
;
Xiufang LIU
;
Fengyu LI
;
Shupeng LUO
;
Hongwei LI
;
Jun SHI
- Publication Type:Journal Article
- Keywords:
Radiation-induced lung injury;
TGF-β1;
TNF-α;
Yiqi Huoxue
- From:
Chinese Journal of Radiological Medicine and Protection
2009;29(4):383-385
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Yiqi Huexue Recipe on the expression of TGF-β1 and TNF-α on serum and lung of rots with radiation-induced lung injury in different radiation time,and discuss the preventative and curative effect and mechanisms of Yiqi Huoxue Recipe.Methods 66 Wistar rats were randomly allocated into irradiation with Yiqi Huoxue Recipe-treated group(A group)irradiation group(B group),and normal control group(C group).The first two groups were irradiated at right hemithorax with a dose of 30 Gy/6 fractions(in 6 weeks).The levels of TGF-β1,and TNF-α on rat serum and lung were measured by ELISA and immunohistochemistry at the end of the 4th,6th,8th,12th,and 26th week after the first irradiation.Results TGF-β1,and TNF-α level on rats serum and lung in irradiation group showed an increase from the 4th week after the first irradiation,peaking at the 12th week,and descending at the 26th week.In A group,the levels of TGF-β1 and TNF-α were significantly lower from the 4th week than those of B group.Conclusions Yiqi Huoxue Recipe could inhibit the excretion of TGF-β1,and TNF-α and might play an important role in the prevention and curation of radiation-induced lung injury,which might be related to inhibiting the expression of TGF-β1 and TNF-α.
