The study of the variation of myocardial glycometabolism and the release of cardiac kinase on isolated rat heart after stimulated with hyperinsulinism
- VernacularTitle:高胰岛素刺激离体大鼠心脏葡萄糖代谢与心肌酶释放的变化
- Author:
Jue WANG
;
Shanglong YAO
;
Jing WU
;
Huarong WANG
- Publication Type:Journal Article
- Keywords:
Insalin/PD;
Heart/DE/PP;
Myocardium/EN;
Glucose/ME
- From:
Journal of Chinese Physician
2009;11(3):318-320
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the variation of myocardial glycometabolism and the release of cardiac creatine kinase and cardiac troponin I on isolated rat heart after stimulated with hyperinsulinism.Methods Thirty Sprague-Dawley rats were random divided into five groups,and their hearts were taken out to be mounted onto a Langendorff perfusion apprartus to perfuse with krebs-Henseleit buffer contained diversity concentration of insulin as 0,10,20,30,50 U/L.The coronary effluent was collected on time,and the concentration of glum was determined to evaluate the glycometabolism of the rat heart,meanwhile the release of cardiac creutine kinase(CK)and cardiac troponin I were detected to evaluate the state of myocardial damage.Results Compared with control group,the rat hearts of the hyperinsulinism group had a significantly depression of glucose uptake and oxidation,and the level of CK and cTn-I in the coronary effluent was increased.Conclusion After the isolated rat hearts were stimulated with hyperinsulinism,the glucose uptake of cadiocyte were suppressed,myocardial insulin resistance appeared,and the rat myocardial got damaged.
